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We use a special solution (cryoprotectant) to protect the sperm from freezing conditions and place it into distinctive containers (cryovials) for freezing. Depending on the size and the quality of the sample, we will use one or more vials per sample. Each vial is labeled with your name, the freeze date and a unique vial number. The vials are slowly cooled, then frozen and stored in our liquid nitrogen tanks. Following freezing of the first sample, a small volume of semen is thawed to determine how well the freezing process worked and the number of motile sperm remaining per vial.